Sunburn Molecule May Provide Insights into Inflammatory Pain Relief

The molecule, called CXCL5, is part of a family of proteins called chemokines, which recruit inflammatory immune cells to the injured tissue, triggering pain and tenderness. This is the first study to reveal this molecule’s role in mediating pain.

The study is planned for publication in the journal Science Translational Medicine.

The research teams, led by Prof. Stephen McMahon and Dr. David Bennett at King’s College London, performed a simple procedure in healthy human volunteers, to expose small patches of their skin to UVB irradiation, creating a small area of sunburn. The treated skin became tender over the following hours, with peak sensory change one to two days later. At this peak, the researchers took small biopsies of the affected skin and analyzed the tissue for hundreds of pain mediators. They found that several of these mediators were overexpressed, so they then examined the biology of these factors in rats to find out whether they were likely to be responsible for driving the pain in the sunburnt skin.

The mediator CXCL5 was considerably overexpressed in the human biopsies and the biology of this chemokine in rats, which suggests it is responsible for a significant amount of sensitivity in the sunburn.

Additional research carried out on the rats revealed that a neutralizing antibody targeting CXCL5 significantly reduced the sensitivity to pain caused by the UVB irradiation.

Prof. Steve McMahon, from the Wolfson Center for Age-Related Diseases at King’s and head of the London Pain Consortium, said, “These findings have shown for the first time the important role of this particular molecule in controlling pain from exposure to UVB irradiation. But this study is not just about sunburn--we hope that we have identified a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis. I’m excited about where these findings could take us in terms of eventually developing a new type of analgesic for people who suffer from chronic pain.”

The researchers noted that not only are the findings of importance for understanding the etiology of pain, but the approach they used by first identifying the mechanisms in humans and then looking at these in preclinical animal models is a novel one in the field of pain research.
Dr. Bennett, Wellcome clinical scientist at King’s and honorary consultant neurologist at King’s College Hospital, said, “Traditionally scientists have first studied the biology of diseases in animal models to identify mechanisms relevant to creating that state. But this often does not translate into effective treatments in the clinic. What we have done is reverse this traditional method by identifying putative mediators in humans first, and then exploring this further in rats. This enabled us to see that the rats’ response to these pain mediators closely parallel those occurring in humans and identify mechanisms of action in the preclinical studies. We intend to extend this approach to other types of pain and in particular to study patients suffering from chronic pain with the hope that this will speed up the process of turning science into effective treatments for patients.”

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